"LDH" Related Products

Lactate Dehydrogenase Activity Assay Kit

Cat.No.: Kit-2257
Product Overview: Lactate Dehydrogenase (LDH: EC 1.1.1.27) is a common enzyme found in wide range of organisms. It catalyzes the interconversion of pyruvate and lactate, with concomitant interconversion of NADH and NAD+. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries. Lactate Dehydrogenase Activity Assay kit provides a quick and easy method for monitoring Lactate Dehydrogenase activity in a wide variety of samples. In this assay, Lactate Dehydrogenase converts lactate into pyruvate and NADH, which reacts with to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, highly sensitive and high-throughput adaptable, and can detect Lactate Dehydrogenase activity as low as 1 μU/ml.
Applications: Measurement of LDH activity in various tissues and cellsEvaluation of tissue damages or cell cytotoxicity.
Storage: -20°C
Shipping: Gel Pack
Size: 500 assays
Kit Components: LDH Assay Buffer; LDH Substrate Mix (Lyophilized); PicoProbe (in DMSO); NADH Standard (Lyophilized); LDH Positive Control
Detection method: Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits: Simple & highly sensitive;
High-throughput adaptable;
Detection Limit: 1 μU/ml

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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