Mouse Anti-Human AGXT Monoclonal Antibody
Cat.No. : | CRP2485 |
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Cat. No. : | CRP2485 |
Antigen Information : | The AGXT gene provides instructions for making a liver enzyme called alanine-glyoxylate aminotransferase. Inside liver cells, this enzyme is found in peroxisomes, structures that contain many different enzymes used to produce energy and the basic materials important for cellular activities. AGXT converts a compound called glyoxylate to glycine, an amino acid that is a building block for making enzymes and other proteins. |
Immunogen : | Recombinant AGXT (330-392aa) purified from E. coli. |
Isotype : | Mouse IgG2b heavy chain and ②light chain. |
Clone : | Anti-human AGXT mAb, clone AT2T4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AGXT protein. |
Concentration : | 1mg/ml. |
Form : | Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
Application : | ELISA, Immunofluorescence. |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Usage : | The antibody has been tested by ELISA and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Immunofluorescence analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500. |
Immunofluorescence Analysis : | Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-AGXT antibody (1:500) with Alexa 488 (Green). |
Tag : | Non |
Gene Name : | AGXT alanine-glyoxylate aminotransferase [ Homo sapiens ] |
Synonyms : | alanine-glyoxylate aminotransferase; AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1; AGXT; serine--pyruvate aminotransferase; serine-pyruvate aminotransferase; serine:pyruvate aminotransferase; alanine--glyoxylate aminotransferase; L-alanine: glyoxylate aminotransferase 1; hepatic peroxisomal alanine:glyoxylate aminotransferase; EC 2.6.1.51,EC 2.6.1.44; Alanine--glyoxylate aminotransferase |
Gene ID : | 189 |
mRNA Refseq : | NM_000030 |
Protein Refseq : | NP_000021 |
MIM : | 604285 |
UniProt ID : | P21549 |
Chromosome Location : | 2q36-q37 |
Pathway : | Alanine, aspartate and glutamate metabolism; Glycine, serine and threonine metabolism; Metabolic pathways; Peroxisome |
Function : | alanine-glyoxylate transaminase activity; amino acid binding; protein binding; protein homodimerization activity; pyridoxal phosphate binding; serine-pyruvate transaminase activity; transferase activity |
Products Types
◆ Recombinant Protein | ||
AGXT-1322M | Recombinant Mouse AGXT Protein (25-414 aa), His-tagged | +Inquiry |
AGXT-314M | Recombinant Mouse AGXT Protein (25-414 aa), His-SUMO-tagged | +Inquiry |
Agxt-555M | Recombinant Mouse Agxt Protein, MYC/DDK-tagged | +Inquiry |
Agxt-504M | Recombinant Mouse Agxt Protein, His-tagged | +Inquiry |
Agxt-505R | Recombinant Rat Agxt Protein, His-tagged | +Inquiry |
◆ Lysates | ||
AGXT-8967HCL | Recombinant Human AGXT 293 Cell Lysate | +Inquiry |
Related Gene
Not For Human Consumption!
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Customer Reviews (5)
Write a reviewThe customer service was fantastic—helpful, friendly, and made the ordering process a breeze!
The efficiency of this protein is outstanding. Saved me a lot of time!
I never knew how much easier my work could be until I tried this protein.
I'm so grateful for this protein – it's a game-changer for my studies!
I can confidently say that this product is worth every penny.
Q&As (10)
Ask a questionAGXT contains a PLP-binding domain, characteristic of aminotransferases, which is critical for its enzymatic activity and substrate specificity.
Yes, mutations in the AGXT gene can cause primary hyperoxaluria type 1 (PH1), a rare autosomal recessive disorder characterized by the overproduction and accumulation of oxalate, resulting in severe kidney and urinary tract complications.
Targeting AGXT enzymatic activity may hold promise for developing therapies to prevent kidney stone formation in non-PH1 patients; however, further research is needed to validate this approach.
AGXT is primarily localized in the peroxisomes, a subcellular organelle responsible for various metabolic processes, including lipid metabolism and detoxification.
Epigenetic modifications, such as DNA methylation or histone acetylation, could potentially regulate AGXT gene expression and activity, affecting oxalate metabolism.
AGXT activity can be regulated by phosphorylation, allosteric modulation, and interaction with other proteins involved in peroxisomal metabolism.
AGXT plays a crucial role in the peroxisomal glyoxylate pathway, converting glyoxylate to glycine to prevent the accumulation of harmful oxalate, which can lead to kidney stone formation.
In PH1 patients, AGXT deficiency impairs the conversion of glyoxylate to glycine, leading to the accumulation of glyoxylate. Excessive glyoxylate is converted to oxalate, which can form kidney stones and cause tissue damage.
AGXT may participate in other cellular processes or metabolic pathways, and its broader functional roles warrant further exploration.
AGXT catalyzes the conversion of glyoxylate and L-alanine to glycine and pyruvate, utilizing PLP as a cofactor.
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