| Antigen Description : |
Interleukin 32 (IL-32),previously known as a transcript (NK4), is produced by mitogen-activated lymphocytes, by IFNgamma-activated epithelial cells or by IL-12 and IL-18-activated NK cells.Its expression is increased following activation of T-cells by mitogens or the activation of NK cells by IL-2.IL-32 activates NF-kappa -B and p38 MAPK cytokine signal pathways. It has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues. IL-32 exists in at least four differentially spliced isoforms (alpha, beta, gamma and delta)with predicted molecular weight: ~26 kD.IL-32alpha is the shortest and most abundant of four potential splice variants of the pro-inflammatory cytokine IL-32.Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32beta, suggesting an intracellular function. |
| Target : |
IL32 |
| Immunogen : |
Recombinant human IL-32 alpha |
| Host : |
Mouse |
| Isotype : |
IgG1, kappa |
| species : |
Human |
| Conjugation : |
Biotin |
| Applications : |
ELISA,Flow Cyt,WB,IFA,IP |
| Preparation : |
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. |
| Usage recommendation : |
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.2-1 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-32 recombinant protein ranging from 2000 to26pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application |
| Concentration : |
0.5 mg/ml |
| Storage : |
The antibody solution should be stored undiluted at 4 °C.Do not freeze. |
| Size : |
50 μg |
