|Product Overview:||Native Bovine FGF2 Protein is purified from Bovine brain tissue.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.|
|Bio-activity:||Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 0.125-0.625 ng/mL.|
|Molecular Mass:||18 kDa|
|Endotoxin:||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE under reducing conditions and visualized by silver stain|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.|
|Gene Name:||fibroblast growth factor 2 [ Bos taurus (cattle) ]|
|Synonyms:||BFGF; FGF-2; HBGF-2|