|Product Overview:||This product is the Fluorescein labeled Datura Stramonium Lectin and has sugar specificity to [GlcNAc]1-3, N-Acetylglucosamine. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.|
|Description:||DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein. The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0.|
|Form:||10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+|
|Bio-activity:||Inhibiting/Eluting Sugar: Chitin Hydrolysate|
|Molecular Mass:||86 Kda|
|Usage:||The recommended concentration range for use is 5-20 µg/ml.|
|Storage:||Refrigerate in the dark. If a precipitate forms upon long-term storage, warm to 37 centigrade. and centrifuge before use.|
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