Native Human C5b,6 Complex Protein


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Native Human C5b,6 Complex Protein

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Cat.No. : C5b6-1537H
Product Overview : Native Human C5b,6 Complex Protein was purified from normal human serum.
Description : C5b,6 is a product of complement activation of C5 and an intermediate in the formation of the C5b-9 membrane attack complex (MAC) of complement.
Source : Normal human serum
Species : Human
Form : Frozen liquid
Bio-activity : >10,000 Units/mg for lysis of sheep erythrocytes.
Molecular Mass : 285,000 Da (3 chains). C5b,6 is composed of two disulfide linked chains from C5b (105,000 and 75,000) and one unlinked chain from C6 (105,000).
Purity : >90% by SDS-PAGE
Unit Definition : One unit of lytic activity is defined as the amount of C5b,6 complex required to yield 50% lysis of 1.5x10^7 sheep erythrocytes when incubated for 30 minutes at 37 centigrade in a total reaction volume of 75 µL GVBE (10 mM EDTA) containing 500 ng C7, 500 ng C8, and 1000 ng C9.
Storage : Store at -70 centigrade or below. Avoid freeze/thaw.
Storage buffer : 10 mM HEPES, 120 mM NaCl, pH 7.2
Official Symbol : C5b,6 Complex

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