Species : |
Human |
Source : |
Human Serum |
Tag : |
Non |
Description : |
C5b,6 is a product of complement activation of C5 and an intermediate in the formation of the C5b-9 membrane attack complex (MAC) of complement. |
Form : |
Frozen liquid |
Bio-activity : |
>10,000 Units/mg for lysis of sheep erythrocytes. |
Molecular Mass : |
285,000 Da (3 chains). C5b,6 is composed of two disulfide linked chains from C5b (105,000 and 75,000) and one unlinked chain from C6 (105,000). |
Purity : |
>90% by SDS-PAGE |
Unit Definition : |
One unit of lytic activity is defined as the amount of C5b,6 complex required to yield 50% lysis of 1.5x10^7 sheep erythrocytes when incubated for 30 minutes at 37 centigrade in a total reaction volume of 75 µL GVBE (10 mM EDTA) containing 500 ng C7, 500 ng C8, and 1000 ng C9. |
Storage : |
Store at -70 centigrade or below. Avoid freeze/thaw. |
Storage buffer : |
10 mM HEPES, 120 mM NaCl, pH 7.2 |