"C5b,6 Complex" Related Products

Native Human C5b,6 Complex Protein

Cat.No.: C5b6-1537H
Product Overview: Native Human C5b,6 Complex Protein was purified from normal human serum.
Description: C5b,6 is a product of complement activation of C5 and an intermediate in the formation of the C5b-9 membrane attack complex (MAC) of complement.
Source: Normal human serum
Species: Human
Form: Frozen liquid
Bio-activity: >10,000 Units/mg for lysis of sheep erythrocytes.
Molecular Mass: 285,000 Da (3 chains). C5b,6 is composed of two disulfide linked chains from C5b (105,000 and 75,000) and one unlinked chain from C6 (105,000).
Purity: >90% by SDS-PAGE
Unit Definition: One unit of lytic activity is defined as the amount of C5b,6 complex required to yield 50% lysis of 1.5x10^7 sheep erythrocytes when incubated for 30 minutes at 37 centigrade in a total reaction volume of 75 µL GVBE (10 mM EDTA) containing 500 ng C7, 500 ng C8, and 1000 ng C9.
Storage: Store at -70 centigrade or below. Avoid freeze/thaw.
Storage buffer: 10 mM HEPES, 120 mM NaCl, pH 7.2
Official Symbol: C5b,6 Complex

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