| Species : |
Human |
| Source : |
Human placenta |
| Description : |
Collagen type III, a fibrous scleroprotein found in bone, cartilage, tendon, bone marrow stroma and other connective tissue, yields gelatin on boiling. Collagen type III scleroprotein is a simple protein found in horny and cartilaginous tissues and in the lens of the eye. In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. |
| Form : |
Liquid in 0.01 N HCl (to be confirmed from label) |
| Endotoxin : |
< 0.05 EU/mg by LAL |
| Applications : |
Typically used as a thin coating on tissue culture surfaces or as a control. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. |
| Notes : |
Human source has been tested and found negative for Hepatitis B, C, Syphilis and immunodeficiency virus-1. |
| Storage : |
Store vial at 2-10°C |
| Concentration : |
To be diluted with 0.01 N HCl to desired concentration |
| Shipping : |
2-10°C |
| EC : |
9007-34-5 |
| Unit definition : |
0.01 N HCl |
| Usage : |
Preperation Procedure: 1. Remove required quantity of collagen from the bottle and dispense into a dilution vessel. 2. Dilute Type III collagen with a 0.01 N HCl to desired concentration. 3. Swirl contents gently until material is completely mixed. |
