Native Porcine ARPC2 Protein (Arp2/3 Protein Complex)

• Cat.No.: ARPC2-01P
• Product Overview: The protein complex has been purified from porcine brain.

Specification

• Species: Porcine
• Source: Brain
• Description: The Arp2/3 protein complex consists of 7 protein subunits, all present in approximately equal stoichiometry. The Arp2/3 complex is a key regulator of actin filament nucleation and the protein subunits are highly evolutionarily conserved.
• Form: Lyophilized powder
• Molecular Mass: The molecular weight of the Arp2/3 complex is 224 kDa.
• Purity: Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20 % polyacrylamide gel. Arp2/3 protein complex is determined to be 90 % pure
• Applications: 1. Positive control for in vitro actin polymerization stimualting compounds
  2. Discovery of Arp2/3 activating or inhibiting compound
  3. Characterization and discovery of Arp2/3 interacting proteins
• Storage Buffer: 20 mM Tris pH 7.5, 25 mM KCl, 1 mM MgCl2, 0.5 mM EDTA, 0.1 mM ATP, 1 % dextran and 5 % sucrose
• Reconstitution: Distilled water

Gene Information

• Gene Name: ARPC2 actin related protein 2/3 complex subunit 2 [ Sus scrofa (pig) ]
• Official Symbol: ARPC2
• Synonyms: ARPC2; actin related protein 2/3 complex subunit 2; actin-related protein 2/3 complex subunit 2
• Gene ID: 100153175
• mRNA Refseq: NM_001190239
• Protein Refseq: NP_001177168
• UniProt ID: A0A4X1UM70

Q&As

Can you provide a characterization of your recombinant ARPC2 protein? Is the recombinant ARPC2 protein functional? What analysis have do you as part of the protein characterization?

The recombinant ARPC2 protein is a modified version of the natural ARPC2 protein, produced through genetic engineering techniques. ARPC2 is a subunit of the ARP2/3 protein complex, which plays a critical role in regulating actin cytoskeletal dynamics. The recombinant ARPC2 protein is typically designed for research purposes and is often used as a tool to study the function and interactions of the ARP2/3 complex. It is generated by introducing the genetic sequence of ARPC2 into a suitable expression system, such as bacteria, yeast, or mammalian cells, that can produce large quantities of the protein. The recombinant ARPC2 protein possesses similar structural and functional characteristics as the native ARPC2 protein. It typically retains the ability to interact with other subunits of the ARP2/3 complex and facilitates the formation of branched actin networks. This essential property allows researchers to investigate the mechanisms underlying actin polymerization and cytoskeletal remodeling in various biological processes. Moreover, the recombinant ARPC2 protein can be tagged with specific markers, such as fluorescent tags or affinity tags, to enable easy detection, purification, and visualization. This versatility enhances the utility of the protein in experimental studies. Overall, the recombinant ARPC2 protein serves as a valuable tool for understanding the cellular processes governed by the ARP2/3 complex and provides insights into its involvement in various physiological and pathological conditions. Our routine QC of ARPC2 protein includes BCA, SDS-PAGE, Western Blot, and Endotoxin detection. Here below are the optional services, case to case. Activity Test; HPLC; SEC; Protein Biotinylation; Protein labeling/conjugation.