"GSN" Related Products


Native Porcine GSN Protein

Cat.No. : GSN-876P
Product Overview : Native Porcine GSN was purified from Porcine Plasma.
Description : Plasma gelsolin has a molecular mass of 83kDa and consists of six homologous subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin regulatory protein, possessing three different actin modulating activities: severing of F-actin, nucleation of actin polymerization and capping of filaments barbed end.
Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and two binding sites for PIP2.
Source : Porcine Plasma
Species : Porcine
Form : 10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and 1% disaccharides, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.
Bio-activity : Fragmentation activity: The fragmentation activity of gelsolin was determined by Ostwald viscometry at 25 centigrade. The specific viscosity of F-actin (1mg/ml) is reduced by ~65% after addition of gelsolin at a molar ratio of 1 : 100.
The gelsolin stock solution was used as described below containing 1% disaccharides. F-actin containing 100mM KCl, 10mM Imidazole pH 7.4, 1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition of gelsolin.
Molecular Mass : 83kDa
Purity : >92% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Storage : Gelsolin is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 5 days. Storage in glycerol at -20 centigrade results in a significant decrase of the activity, and has to determined individually.
Shipping : At ambient temperature. Upon delivery store at -70 centigrade.
Gene Name : GSN gelsolin [ Sus scrofa ]
Official Symbol : GSN
Synonyms : gelsolin
Gene ID : 396874

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