| Species : |
Microorganism |
| Source : |
Microorganism |
| Tag : |
Non |
| Description : |
Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. |
| Predicted MolecularMass : |
68kDa |
| Isoelectic point : |
5.2 |
| Michaelis constant : |
1.1mM (ADP); 2.2mM(PEP) |
| Optimum pH : |
7.5 |
| Optimum temperature : |
80°C |
| pH Stability : |
5.0~10.0 (37°C, 20hr) |
| Thermal Stability : |
< 60°C (pH8.5, 20min) |
| Inhibitors : |
Ag+, Hg2+ |
| Specific Activity : |
≥ 300U/mg protein |
| Unit definition : |
One unit will convert one micromole of phosphoenolpyruvate to pyruvate per min at pH 7.2 at 30°C. |
| Storage : |
-20°C |
| PreparationInstructions : |
The enzyme is reconstituted in 50mM Tris-HCl buffer, pH 8.5 for activity assay. |
| Background : |
| Introduction | The protein is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). | | Keywords | Pyruvate kinase; EC: 2.7.1.40; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; OPA interacting protein 3; Opa interactivity protein; PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase isozyme R/L; Pyruvate kinase liver and RBC; Pyruvate kinase muscle isozyme; R type/L type pyruvate kinase; RPK; THBP 1; Thyroid hormone binding protein cytosolic |
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