Rabbit Anti-Human Carbonic Anhydrase XIV Monoclonal Antibody

• Cat.No.: CAB11486RH
• Product Overview: Rabbit monoclonal antibody to human carbonic anhydrase XIV.

Specification

• Antigen Description: Carbonic anhydrases (CAs) are zinc-binding metalloenzymes that catalyze the reversible hydration of carbon dioxide to form HCO3- and protons. Fifteen CA isoforms have been reported sofar. CA I, CA II, CA III, CA VII, and CA XIII are cytosolic enzymes, whereas CA IV, CA IX, CA XII, CA XIV, and CA XV are membrane-associated isoforms. CA XIV is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C-terminal segment with several potential phosphorylation sites. The CA XIV protein is expressed in the brain on plasma membranes of neuronal bodies and axons, in the kidney preferentially on the apical membranes of proximal tubules, and in the liver on the plasma membranes of hepatocytes. CA14, together with CA4, play important roles in the regulation of intracellular pH in hippocampal neurons by facilitating AE3-mediated Cl--HCO3- exchange.
• Specificity: Human Carbonic Anhydrase XIV / CA14. No cross-reactivity in ELISA with Human CARPX, Human CA12, Mouse CA14.
• Immunogen: Recombinant Human Carbonic Anhydrase XIV / CA14 Protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Human
• Clone: 516
• Applications: Western Blot; ELISA; Immunohistochemistry
• Dilution: Immunochemistry : IHC-P: 2 - 8 μg/mL.Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human CA14 in WB. Using a DAB detection system, the detection limit for Human CA14 is approximately 0.25 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect Human CA14. The detection limit for Human CA14 is approximately 0.00245 ng/well.
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human Carbonic Anhydrase XIV / CA14.
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: CA14 carbonic anhydrase XIV [ Homo sapiens ]
• Official Symbol: CA14
• Synonyms: CA14; carbonic anhydrase XIV; carbonic anhydrase 14; CA-XIV; carbonic dehydratase; carbonate dehydratase XIV; CAXiV
• Gene ID: 23632
• mRNA Refseq: NM_012113
• Protein Refseq: NP_036245
• MIM: 604832
• UniProt ID: Q9ULX7
• Chromosome Location: 1q21
• Function: carbonate dehydratase activity; lyase activity; metal ion binding

Reviews

08/19/2022

Reliable source, ensures reproducibility in our research.

11/10/2020

Efficient services, optimize our protein-related studies.

10/01/2020

Responsive customer support, aids in problem-solving effectively.

Q&As

Are there any known small molecule modulators specific to CA14's activity?

Specific modulators can be identified through high-throughput screening assays targeting CA14 and validated using in vitro and in vivo models.

What unique functional attributes differentiate CA14 from other carbonic anhydrase isoforms?

CA14's unique functional attributes can be discerned using comparative enzymatic assays and structural analyses against other isoforms.

How do changes in cellular pH or ion concentrations influence CA14 activity?

Influence of pH or ions on CA14 can be analyzed using enzymatic assays under varying conditions and ion-sensitive probes.

Are there any known disease associations where CA14 mutations or dysregulation play a pivotal role?

Disease associations can be determined through genetic studies, patient tissue analyses, and literature reviews focusing on CA14's relevance in pathological conditions.

In what tissues or cell types is CA14 predominantly expressed, and how does this correlate with its physiological role?

Predominant tissue or cell-type expression can be determined using tissue microarrays, qPCR, or proteomic databases, correlating findings with potential physiological implications.

What are the consequences of CA14 overexpression or knockdown in cellular and organismal models?

Consequences of overexpression or knockdown can be assessed using phenotypic analyses, cellular viability assays, and in vivo studies in organisms with altered CA14 expression.

Are there specific cellular pathways where CA14 plays a crucial modulatory role?

CA14's role in cellular pathways can be explored using pathway enrichment analyses, proteomic studies, and pathway-specific assays in CA14-modified cells.