Recombinant Mouse CA14 cell lysate

• Cat.No.: CA14-3064MCL
• Product Overview: Mouse CA14 / Car14 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Car14 carbonic anhydrase 14 [ Mus musculus ]
• Official Symbol: Car14
• Synonyms: Ca14; AW536446; carbonic anhydrase 14; CA XIV; CA-XIV; carbonic anhydrase XIV; carbonate dehydratase XIV
• Gene ID: 23831
• mRNA Refseq: NM_011797
• Protein Refseq: NP_035927
• UniProt ID: Q9WVT6
• Chromosome Location: 3 F2.1; 3
• Pathway: Metabolism, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Nitrogen metabolism, conserved biosystem
• Function: carbonate dehydratase activity; carbonate dehydratase activity; lyase activity

Reviews

08/19/2022

Reliable source, ensures reproducibility in our research.

11/10/2020

Efficient services, optimize our protein-related studies.

10/01/2020

Responsive customer support, aids in problem-solving effectively.

Q&As

Are there any known small molecule modulators specific to CA14's activity?

Specific modulators can be identified through high-throughput screening assays targeting CA14 and validated using in vitro and in vivo models.

What unique functional attributes differentiate CA14 from other carbonic anhydrase isoforms?

CA14's unique functional attributes can be discerned using comparative enzymatic assays and structural analyses against other isoforms.

How do changes in cellular pH or ion concentrations influence CA14 activity?

Influence of pH or ions on CA14 can be analyzed using enzymatic assays under varying conditions and ion-sensitive probes.

Are there any known disease associations where CA14 mutations or dysregulation play a pivotal role?

Disease associations can be determined through genetic studies, patient tissue analyses, and literature reviews focusing on CA14's relevance in pathological conditions.

In what tissues or cell types is CA14 predominantly expressed, and how does this correlate with its physiological role?

Predominant tissue or cell-type expression can be determined using tissue microarrays, qPCR, or proteomic databases, correlating findings with potential physiological implications.

What are the consequences of CA14 overexpression or knockdown in cellular and organismal models?

Consequences of overexpression or knockdown can be assessed using phenotypic analyses, cellular viability assays, and in vivo studies in organisms with altered CA14 expression.

Are there specific cellular pathways where CA14 plays a crucial modulatory role?

CA14's role in cellular pathways can be explored using pathway enrichment analyses, proteomic studies, and pathway-specific assays in CA14-modified cells.