Recombinant 2019-nCoV (2019 novel coronavirus) Spike protein S1, His/Avi-tagged

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Recombinant 2019-nCoV (2019 novel coronavirus) Spike protein S1, His/Avi-tagged

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Cat.No. : Spike-200V
Product Overview : Recombinant 2019-nCoV (2019 novel coronavirus) Spike protein S1 (Gln14-Arg683) was expressed from mammalian with a His tag and Avi at the C-terminal.
Description : The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensinconverting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source : Mammalian cells
Species : 2019-nCoV
Tag : His/Avi
Form : Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Molecular Mass : The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Protein length : 670
Endotoxin : Less than 1 EU per ug by the LAL method.
Purity : >95% as determined by Bis-Tris PAGE; >95% as determined by HPLC.
Storage : The product should be stored at -70 centigrade. Please do not repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Warning : For Research Use Only.
Synonyms : BHT41_gp02
Tris-Bis PAGE of Spike-200V :

Recombinant 2019-nCoV Spike S1 protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

HPLC Data of Spike-200V :

The purity of 2019-nCoV Spike S1 is greater than 95% as determined by SEC-HPLC.

ELISA of Spike-200V :

Immobilized nCov-S1 at 0.5μg/ml(100μl/Well). Dose response curve for Biotinylated Human ACE2 with the EC50 of 0.2μg/ml determined by ELISA.

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