Recombinant 2019-nCoV (2019 novel coronavirus) Spike protein S1, His/Avi-tagged
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|Product Overview :||Recombinant 2019-nCoV (2019 novel coronavirus) Spike protein S1 (Gln14-Arg683) was expressed from mammalian with a His tag and Avi at the C-terminal.|
|Description :||The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensinconverting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.|
|Source :||Mammalian cells|
|Form :||Supplied as 0.22μm filtered solution in PBS (pH 7.4).|
|Molecular Mass :||The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.|
|Protein length :||670|
|Endotoxin :||Less than 1 EU per ug by the LAL method.|
|Purity :||>95% as determined by Bis-Tris PAGE; >95% as determined by HPLC.|
|Storage :||The product should be stored at -70 centigrade. Please do not repeated freeze-thaw cycles.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Warning :||For Research Use Only.|
|Tris-Bis PAGE of Spike-200V :||
Recombinant 2019-nCoV Spike S1 protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
|HPLC Data of Spike-200V :||
The purity of 2019-nCoV Spike S1 is greater than 95% as determined by SEC-HPLC.
|ELISA of Spike-200V :||
Immobilized nCov-S1 at 0.5μg/ml(100μl/Well). Dose response curve for Biotinylated Human ACE2 with the EC50 of 0.2μg/ml determined by ELISA.
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