Recombinant 2019-nCoV (2019 novel coronavirus) Spike protein S1, His/Avi-tagged


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Recombinant 2019-nCoV (2019 novel coronavirus) Spike protein S1, His/Avi-tagged

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Cat.No. : Spike-200V
Product Overview : Recombinant 2019-nCoV (2019 novel coronavirus) Spike protein S1 (Gln14-Arg683) was expressed from mammalian with a His tag and Avi at the C-terminal.
Description : The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensinconverting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source : Mammalian cells
Species : 2019-nCoV
Tag : His/Avi
Form : Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Molecular Mass : The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Protein length : 670
Endotoxin : Less than 1 EU per ug by the LAL method.
Purity : >95% as determined by Bis-Tris PAGE; >95% as determined by HPLC.
Storage : The product should be stored at -70 centigrade. Please do not repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Warning : For Research Use Only.
Synonyms : BHT41_gp02
Tris-Bis PAGE of Spike-200V :

Recombinant 2019-nCoV Spike S1 protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

HPLC Data of Spike-200V :

The purity of 2019-nCoV Spike S1 is greater than 95% as determined by SEC-HPLC.

ELISA of Spike-200V :

Immobilized nCov-S1 at 0.5μg/ml(100μl/Well). Dose response curve for Biotinylated Human ACE2 with the EC50 of 0.2μg/ml determined by ELISA.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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