FGF4-86H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Active Human FGF4 Protein with His tag (C-ter) was expressed in E. coli.
Description :
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. [provided by RefSeq, Jul 2008]
Source :
E. coli
Species :
Human
Tag :
His(C-ter)
Form :
Powder
Bio-activity :
Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 2.5 ng/mL. The specific activity of recombinant human FGF-4 is > 4 x 10^5 IU/mg.
Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
Purity :
> 95% (by SDS-PAGE)
Applications :
SDS-PAGE
Notes :
For laboratory research only, not for drug, diagnostic or other use.
Storage :
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Storage Buffer :
PBS (pH 8.0) and 0.1% Sarkosyl.
Reconstitution :
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.