Description : |
The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. [provided by RefSeq, Jul 2008] |
Source : |
E. coli |
Species : |
Human |
Tag : |
His(C-ter) |
Form : |
Powder |
Bio-activity : |
Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 49 ng/mL. |
AA Sequence : |
MQPRSPKSKRKGQGRPGPLAPGPHQ VPLDLVSRMKPYARMEEYERNIEEM VAQLRNSSELAQRKCEVNLQLWMSN KRSLSPWGYSINHDPSRIPVDLPEA RCLCLGCVNPFTMQEDRSMVSVPVF SQVPVRRRLCPPPPRTGPCRQRAVM ETIAVGCTCIF |
Endotoxin : |
Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test. |
Purity : |
> 98% (by SDS-PAGE) |
Applications : |
SDS-PAGE |
Notes : |
For laboratory research only, not for drug, diagnostic or other use. |
Storage : |
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
Storage Buffer : |
PBS (pH 8.0) |
Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |