IL32-190H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Active Human IL32 Protein with His tag (C-ter) was expressed in E. coli.
Description :
This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Source :
E. coli
Species :
Human
Tag :
His(C-ter)
Form :
Powder
Bio-activity :
Determined by its ability to induce TNF alpha secretion in RAW264.7 cells. The ED50 for this effect is < 10 μg/mL.
Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
Purity :
> 98% (by SDS-PAGE)
Applications :
SDS-PAGE
Notes :
For laboratory research only, not for drug, diagnostic or other use.
Storage :
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Storage Buffer :
PBS (pH 8.0)
Reconstitution :
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.