Active Recombinant Mouse Il17f protein, His-tagged

• Cat.No.: Il17f-152M
• Product Overview: Recombinant Mouse Il17f protein(Met1-Ala161), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-161 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1. Immobilized recombinant Mouse IL-17F at 2μg/mL (100μL/well) can bind recombinant Mouse IL17RA with a linear range of 31. 25-163ng/mL. 2. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFα. The ED50 for this effect is 10.18-140.72 ng/mL, corresponding to a specific activity of 2.46×10^4~9.82×10^4 units/mg.
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Il17f interleukin 17F [ Mus musculus ]
• Official Symbol: Il17f
• Synonyms: IL17F; interleukin 17F; interleukin-17F; C87042; IL-17F
• Gene ID: 257630
• mRNA Refseq: NM_145856
• Protein Refseq: NP_665855