Recombinant Human IL17F cell lysate

• Cat.No.: IL17F-2045HCL
• Product Overview: Human IL-17F / Interleukin-17F derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: IL17F interleukin 17F [ Homo sapiens ]
• Official Symbol: IL17F
• Synonyms: IL17F; interleukin 17F; interleukin-17F; IL 17F; ML 1; ML1; IL-24; cytokine ML-1; mutant IL-17F; interleukin-24; ML-1; CANDF6; IL-17F
• Gene ID: 112744
• mRNA Refseq: NM_052872
• Protein Refseq: NP_443104
• MIM: 606496
• UniProt ID: Q96PD4
• Chromosome Location: 6p12
• Pathway: IL23-mediated signaling events, organism-specific biosystem
• Function: cytokine activity; cytokine binding; protein homodimerization activity

Reviews

11/01/2022

High specificity.

02/23/2020

Good for receptor studies.

02/05/2018

Potent in anti-bacterial assays.

Q&As

What techniques are used to assess IL17F's involvement in cancer development and its potential as a therapeutic target?

Tumor xenografts, immune cell infiltration analysis, and therapeutic antibody testing assess IL17F's role in cancer.

What methodologies are employed to explore IL17F's role in promoting neutrophil recruitment and activation at mucosal sites?

Neutrophil chemotaxis assays, in vivo neutrophil tracking, and immune cell activation analysis explore IL17F's impact on neutrophil functions.

How do researchers differentiate the functional roles of IL17F from other IL17 family members in immune responses?

Genetic knockout models, functional assays, and gene expression analyses distinguish IL17F's unique roles.

How is the interplay between IL17F and gut microbiota investigated, and what are the implications for intestinal health?

Microbiota composition analysis, germ-free models, and immune cell profiling investigate IL17F-microbiota interactions.

Could you elaborate on the strategies employed to investigate IL17F's contribution to autoimmune diseases using experimental models and patient samples?

Animal disease models, patient samples, and immunological assays elucidate IL17F's contribution to autoimmune diseases.

What experimental techniques are utilized to dissect the complex heterodimerization of IL17F with IL17A and its impact on signaling pathways?

Co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and pathway-specific assays elucidate IL17F's heterodimerization and signaling.

How do researchers investigate the potential synergistic or antagonistic effects of IL17F on tissue inflammation in comparison to other cytokines?

Comparative cytokine profiling, inflammatory assays, and pathway analysis dissect IL17F's interplay with other immune mediators.

What molecular approaches are used to study IL17F's involvement in chronic inflammatory conditions, such as asthma and inflammatory bowel diseases?

Allergen challenges, inflammatory disease models, and gene expression profiling probe IL17F's involvement in chronic conditions.

Can you explain the experimental designs used to study the impact of IL17F on barrier function in epithelial tissues?

Epithelial barrier permeability assays, tissue section analysis, and cell culture models study IL17F's effects on barrier integrity.

How do researchers explore the effects of IL17F modulation on tissue repair and regeneration in experimental models?

Wound healing assays, tissue regeneration models, and histological analyses examine IL17F's effects on tissue repair.