Recombinant Human IL17F cell lysate

Cat.No. : IL17F-2045HCL
Product Overview : Human IL-17F / Interleukin-17F derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL17F interleukin 17F [ Homo sapiens ]
Official Symbol : IL17F
Synonyms : IL17F; interleukin 17F; interleukin-17F; IL 17F; ML 1; ML1; IL-24; cytokine ML-1; mutant IL-17F; interleukin-24; ML-1; CANDF6; IL-17F;
Gene ID : 112744
mRNA Refseq : NM_052872
Protein Refseq : NP_443104
MIM : 606496
UniProt ID : Q96PD4
Chromosome Location : 6p12
Pathway : IL23-mediated signaling events, organism-specific biosystem;
Function : cytokine activity; cytokine binding; protein homodimerization activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques are used to assess IL17F's involvement in cancer development and its potential as a therapeutic target? 03/05/2022

Tumor xenografts, immune cell infiltration analysis, and therapeutic antibody testing assess IL17F's role in cancer.

What methodologies are employed to explore IL17F's role in promoting neutrophil recruitment and activation at mucosal sites? 04/05/2021

Neutrophil chemotaxis assays, in vivo neutrophil tracking, and immune cell activation analysis explore IL17F's impact on neutrophil functions.

How do researchers differentiate the functional roles of IL17F from other IL17 family members in immune responses? 02/04/2021

Genetic knockout models, functional assays, and gene expression analyses distinguish IL17F's unique roles.

How is the interplay between IL17F and gut microbiota investigated, and what are the implications for intestinal health? 07/21/2020

Microbiota composition analysis, germ-free models, and immune cell profiling investigate IL17F-microbiota interactions.

Could you elaborate on the strategies employed to investigate IL17F's contribution to autoimmune diseases using experimental models and patient samples? 05/27/2020

Animal disease models, patient samples, and immunological assays elucidate IL17F's contribution to autoimmune diseases.

What experimental techniques are utilized to dissect the complex heterodimerization of IL17F with IL17A and its impact on signaling pathways? 04/09/2020

Co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and pathway-specific assays elucidate IL17F's heterodimerization and signaling.

How do researchers investigate the potential synergistic or antagonistic effects of IL17F on tissue inflammation in comparison to other cytokines? 12/21/2019

Comparative cytokine profiling, inflammatory assays, and pathway analysis dissect IL17F's interplay with other immune mediators.

What molecular approaches are used to study IL17F's involvement in chronic inflammatory conditions, such as asthma and inflammatory bowel diseases? 03/31/2018

Allergen challenges, inflammatory disease models, and gene expression profiling probe IL17F's involvement in chronic conditions.

Can you explain the experimental designs used to study the impact of IL17F on barrier function in epithelial tissues? 06/24/2017

Epithelial barrier permeability assays, tissue section analysis, and cell culture models study IL17F's effects on barrier integrity.

How do researchers explore the effects of IL17F modulation on tissue repair and regeneration in experimental models? 03/21/2017

Wound healing assays, tissue regeneration models, and histological analyses examine IL17F's effects on tissue repair.

Customer Reviews (3)

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Reviews
11/01/2022

    High specificity.

    02/23/2020

      Good for receptor studies.

      02/05/2018

        Potent in anti-bacterial assays.

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