Recombinant Activity Human METTL3/METTL14 Complex Protein, GST-tagged

• Cat.No.: METTL3-610H
• Product Overview: Full-length recombinant human METTL3/METTL14 complex was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
• Source: Insect cell
• Species: Human
• Tag: GST
• Molecular Mass: 100 kDa (METTL3) and 84 kDa (METTL14)
• Purity: >90 %
• Applications: Methyltransferase Assay
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Gene Information

• Gene Name: METTL3 methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: METTL3
• Synonyms: METTL3; methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit; M6A; IME4; Spo8; MT-A70; hMETTL3; N6-adenosine-methyltransferase catalytic subunit; N6-adenosine-methyltransferase 70 kDa subunit; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; methyltransferase like 3; methyltransferase-like protein 3; EC 2.1.1.348
• Gene ID: 56339
• mRNA Refseq: NM_019852
• Protein Refseq: NP_062826
• MIM: 612472
• UniProt ID: Q86U44