Recombinant Arabidopsis Thaliana AUR1 Protein (1-294 aa), His-SUMO-Myc-tagged

• Cat.No.: AUR1-2173A
• Product Overview: Recombinant Arabidopsis Thaliana (Mouse-ear cress) AUR1 Protein (1-294 aa) is produced by E. coli expression system. This protein is fused with a 10xHis-SUMO tag at the N-terminal and a Myc tag at the C-terminal. Protein Description: Full Length.

Specification

• Description: Phosphorylates specifically 'Ser-10' of histone H3 in vitro and colocalizes with phosphorylated histone H3 during mitosis. Associates with cytoskeletal structures that are necessary for cytokinesis and with the microtubule spindle. Colocalizes also with gamma-tubulin and function in microtubule organizing centers (MTOCs). In contrast with the mammalian B-type Aurora, AUR1 has no kinase activity toward 'Ser-28' of histone H3.
• Source: E. coli
• Species: Arabidopsis Thaliana
• Tag: His-SUMO-Myc
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 54.0 kDa
• Protein length: 1-294 aa
• AA Sequence: MAIPTETQHQEKEASDASAAAAQKR WTLSDFDIGKPLGRGKFGHVYLARE KRSNHVVALKVLFKSQLQQSQVEHQ LRREVEIQSHLRHPNILRLYGYFYD QKRVYLILEYAARGELYKDLQKCKY FSERRAATYVASLARALIYCHGKHV IHRDIKPENLLIGAQGELKIADFGW SVHTFNRRRTMCGTLDYLPPEMVES VEHDASVDIWSLGILCYEFLYGVPP FEAMEHSDTYRRIVQVDLKFPPKPI ISASAKDLISQMLVKESSQRLPLHK LLEHPWIVQNADPSGIYRV
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: AUR1 ataurora1 [ Arabidopsis thaliana (thale cress) ]
• Official Symbol: AUR1
• Synonyms: AUR1; AtAUR1; ataurora1; T16I18.40; T16I18_40
• Gene ID: 829419
• mRNA Refseq: NM_119436
• Protein Refseq: NP_195009
• UniProt ID: Q9M077

Reviews

12/08/2019

The production process of AUR1 uses a closed biological reaction system, which reduces the generation of waste and pollution to the environment.

10/13/2019

Catalytic activity of AUR1 is very excellent, and it can be widely used in various chemical reactions, which has high practical value.

05/19/2019

This stability of AUR1 is very good, and its properties remain stable even after prolonged storage, which shows its excellent storage stability.

Q&As

What are the characteristics of AURgene promoter sequence?

The promoter sequence of the AUR1 gene may contain transcription factor binding sites related to cell cycle regulation and phosphatidylinositol metabolism.

What external factors regulate AURexpression?

AUR1 gene expression may be regulated by the cell cycle, as well as by signaling pathways related to phosphatidylinositol metabolism.

What is the regulatory mechanism of AURgene?

AUR1 gene expression is regulated by yeast cell cycle regulators and other transcription factors to ensure the right timing and conditions for expression.

Which phenotypes are associated with AURmutations?

Mutations in the AUR1 gene may cause defects in yeast in the synthesis of phosphatidylinositol required for cell membranes, thereby affecting membrane assembly and function.

In what biological processes does the AURgene function?

The AUR1 gene is involved in the inositol metabolic pathway in yeast, and in particular plays an important role in the synthesis of phosphatidylinositol required for cell membranes.

In which tissues is AURgene expressed?

The AUR1 gene is mainly expressed in the cells of yeast, especially in tissues and cells related to membrane synthesis.