Recombinant Bacillus subtilis AROF protein, His-tagged

• Cat.No.: AROF-1626B
• Product Overview: Recombinant Bacillus subtilis AROF full length or partial length protein was expressed.

Specification

• Source: E. coli or Yeast
• Species: Bacillus subtilis
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 80% by SDS-PAGE
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage buffer: PBS buffer

Gene Information

• Gene Name: aroF chorismate synthase [ Bacillus subtilis subsp. subtilis str. 168 ]
• Official Symbol: AROF
• Synonyms: AROF; chorismate synthase
• Gene ID: 939000
• UniProt ID: P31104

Reviews

08/13/2022

By utilizing the AROF protein in my experiments, I can trust that I am working with a product of the highest caliber, backed by a company dedicated to delivering exceptional quality and support.

12/30/2020

Great performance in ELISA.

10/11/2017

Its purity and functionality ensure precise and reproducible results, enabling me to achieve my research objectives with confidence.

Q&As

Can the AROF protein be influenced by lifestyle or environmental factors?

Yes, lifestyle and environmental factors can potentially influence the activity of the AROF protein. For instance, obesity and excess body fat have been associated with increased AROF expression and higher estrogen levels due to adipose tissue being a site of estrogen synthesis. Conversely, exposure to certain endocrine-disrupting chemicals or environmental pollutants has been shown to interfere with AROF activity and estrogen production.

What are the consequences of AROF protein deficiency?

AROF protein deficiency can have significant physiological consequences. In females, it can lead to impaired sexual development and infertility, as estrogen plays a vital role in the maturation of the reproductive system. In males, AROF deficiency may cause delay or abnormality in sexual development. Moreover, AROF deficiency can result in hormonal imbalances, affecting bone health, lipid metabolism, and other estrogen-dependent processes.

Are there any medications or therapeutic interventions that target the AROF protein?

Yes, there are medications and therapeutic interventions that target the AROF protein. Aromatase inhibitors (AIs) are drugs often used in the treatment of estrogen receptor-positive breast cancer. These inhibitors specifically target and block the activity of the AROF protein, reducing the production of estrogen. By inhibiting AROF, these medications help to prevent estrogen-driven tumor growth and progression.

What are some diseases or conditions associated with dysregulation of the AROF protein?

Dysregulation of the AROF protein can contribute to several diseases and conditions. For example, excessive activity of AROF can result in increased estrogen production and has been linked to estrogen-dependent cancers such as breast, ovarian, and endometrial cancer. Conversely, decreased AROF activity or deficiency can lead to conditions like aromatase excess syndrome (AEXS) or aromatase deficiency, which are characterized by hormonal imbalances and various symptoms related to estrogen deficiency.

Are there any known mutations in the AROF gene that affect its function?

Yes, mutations in the AROF gene have been identified and can impact its function. These mutations can lead to altered enzyme activity or reduced protein stability, resulting in impaired estrogen synthesis. Such mutations may cause disorders related to impaired sexual development, fertility issues, or other complications associated with estrogen deficiency.

Is the AROF protein exclusively involved in estrogen synthesis?

While the AROF protein is primarily known for its involvement in estrogen synthesis, it may have additional functions beyond this role. There is evidence suggesting that AROF could have non-enzymatic functions, such as serving as a regulator of gene expression or participating in protein-protein interactions. Further research is needed to fully understand the diverse functions of the AROF protein.