Recombinant Baker's yeast ACC1 protein, His-tagged

• Cat.No.: ACC1-3680B
• Product Overview: Recombinant Baker's yeast ACC1 protein(Q00955)(217-775aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Baker's yeast
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 67.6 kDa
• Protein length: 217-775aa
• AA Sequence: TGLVSVDDDIYQKGCCTSPEDGLQK AKRIGFPVMIKASEGGGGKGIRQVE REEDFIALYHQAANEIPGSPIFIMK LAGRARHLEVQLLADQYGTNISLFG RDCSVQRRHQKIIEEAPVTIAKAET FHEMEKAAVRLGKLVGYVSAGTVEY LYSHDDGKFYFLELNPRLQVEHPTT EMVSGVNLPAAQLQIAMGIPMHRIS DIRTLYGMNPHSASEIDFEFKTQDA TKKQRRPIPKGHCTACRITSEDPND GFKPSGGTLHELNFRSSSNVWGYFS VGNNGNIHSFSDSQFGHIFAFGENR QASRKHMVVALKELSIRGDFRTTVE YLIKLLETEDFEDNTITTGWLDDLI THKMTAEKPDPTLAVICGAATKAFL ASEEARHKYIESLQKGQVLSKDLLQ TMFPVDFIHEGKRYKFTVAKSGNDR YTLFINGSKCDIILRQLSDGGLLIA IGGKSHTIYWKEEVAATRLSVDSMT TLLEVENDPTQLRTPSPGKLVKFLV ENGEHIIKGQPYAEIEVMKMQMPLV SQENGIVQLLKQPGSTIVAGDIMAI MTLDDPSKV
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Reviews

10/12/2020

I saved a lot of time and resources by purchasing the recombinant Yeast ACC1 protein from Creative BioMart instead of making it myself. The cost-effectiveness is a major benefit.

09/18/2020

I had a great experience purchasing the recombinant ACC1 protein from Creative BioMart. Their customer service was exceptional and the product performed flawlessly in my experiments.

06/18/2020

I highly recommend the recombinant Baker's yeast ACC1 protein. Its high quality and reliable performance make it an essential part of my research toolkit.

Q&As

Are there any discounts available?

We are offering a 10% discount on all products for a limited time.

Can Recombinant ACC1 Protein be used to generate antibodies for research or diagnostic applications?

Yes, it can be used to generate antibodies against ACC1.

What is the recommended method for determining the recombinant ACC1 protein concentration?

Our recombinant protein products have been tested for concentration. Of course, you can also determine the concentration again in experiments such as spectrophotometry, BCA assay, or Bradford assay.

Can the recombinant ACC1 protein be used in structural biology studies?

Yes, it can be used in structural biology studies, such as X-ray crystallography, to determine its three-dimensional structure.

Can Recombinant ACC1 Protein be used in high-throughput screening assays?

Yes, Recombinant ACC1 Protein can be used in high-throughput screening assays for drug discovery or other applications. However, researchers may need to optimize the assay conditions, such as the substrate concentration or reaction time, to ensure that the results are reproducible and specific.

Is there any established method for quantifying the activity of recombinant ACC1 protein?

Several methods can be used to quantify the activity of recombinant ACC1 protein, depending on the specific assay and the requirements of the experiment. These may include radioactive substrate assays, colorimetric or fluorescent assays, or mass spectrometry-based assays.

Can Recombinant ACC1 Protein be used in conjunction with other proteins or reagents?

Yes, it can potentially be used in conjunction with other proteins or reagents in various applications such as protein-protein interaction studies, biochemical assays, or diagnostic assays. However, it's important to ensure that any potential interactions or interferences are taken into account and that the protein maintains its activity and stability in the presence of other components.

Can recombinant ACC1 protein be customized or modified to meet specific research needs?

Yes, we can offer customization or modification services, such as site-directed mutagenesis or fusion with other tags or proteins.

I plan to buy Recombinant Yeast ACC1 protein (ACC1-3683Y), is there any discount?

Discounts are available for first-time purchases and holidays. For specific discounts, please contact our customer service staff.

Is there any established method for measuring the kinetic parameters of recombinant ACC1 protein?

Several methods can be used to measure the kinetic parameters of recombinant ACC1 protein, such as the Michaelis-Menten constant (Km) and the maximal reaction rate (Vmax). These may include substrate concentration curves, Lineweaver-Burk plots, or other methods that allow for the determination of the rate of enzyme activity at varying substrate concentrations.