Recombinant bovine NOS3 Protein, His-tagged

• Cat.No.: NOS3-66B
• Product Overview: Recombinant bovine NOS3 protein without tag was expressed in Sf9 cells.

Specification

• Species: Bovine
• Source: Insect Cells
• Description: Nitric oxide synthase (NOS) is responsible for the biosynthesis of NO from L-arginine. Constitutively expressed NOS is found in brain (nNOS) and endothelial cells (eNOS), whereas macrophages can be induced by cytokines or endotoxin to express NOS (iNOS). eNOS undergoes several post-translational modifications, including myristolation and palmitoylation, which causes the enzyme to become membrane-associated.
• Molecular Mass: 135 kDa
• Purity: cell lysate 100,000 x g supernatant
• Unit Definition: One unit of enzyme produces 1 nanomole of nitric oxide per minute at 37 centigrade in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/mL calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.
• Stability: ≥ 6 months
• Storage: Store it at -80 centigrade
• Storage Buffer: 50 mM HEPES, pH 7.4, containing 10% glycerol, 5 mM CHAPS, and 100 μM DTT

Gene Information

• Gene Name: NOS3 nitric oxide synthase 3 [ Bos taurus (cattle) ]
• Official Symbol: NOS3
• Synonyms: NOS3; nitric oxide synthase 3; nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; eNOS; endothelial NOS; endothelial nitric oxide synthase; nitric oxide synthase 3 (endothelial cell)
• Gene ID: 287024
• mRNA Refseq: NM_181037
• Protein Refseq: NP_851380
• UniProt ID: P29473