Recombinant bovine NOS3 Protein, His-tagged NOS3-66B

Recombinant bovine NOS3 Protein, His-tagged


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Recombinant bovine NOS3 Protein, His-tagged

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Cat.No. : NOS3-66B
Product Overview : Recombinant bovine NOS3 protein without tag was expressed in Sf9 cells.
Description : Nitric oxide synthase (NOS) is responsible for the biosynthesis of NO from L-arginine. Constitutively expressed NOS is found in brain (nNOS) and endothelial cells (eNOS), whereas macrophages can be induced by cytokines or endotoxin to express NOS (iNOS). eNOS undergoes several post-translational modifications, including myristolation and palmitoylation, which causes the enzyme to become membrane-associated.
Source : Insect cells
Species : Bovine
Molecular Mass : 135 kDa
Purity : cell lysate 100,000 x g supernatant
Unit Definition : One unit of enzyme produces 1 nanomole of nitric oxide per minute at 37 centigrade in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/mL calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.
Stability : ≥ 6 months
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM HEPES, pH 7.4, containing 10% glycerol, 5 mM CHAPS, and 100 μM DTT
Gene Name : NOS3 nitric oxide synthase 3 [ Bos taurus (cattle) ]
Official Symbol : NOS3
Synonyms : NOS3; nitric oxide synthase 3; nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; eNOS; endothelial NOS; endothelial nitric oxide synthase; nitric oxide synthase 3 (endothelial cell)
Gene ID : 287024
mRNA Refseq : NM_181037
Protein Refseq : NP_851380
UniProt ID : P29473

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