Recombinant Canine CD274 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD274-1058CAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant canine CD274 (XP_541302.4) (Met1-Arg236) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Dog
• Source: HEK293
• Tag: Fc
• Protein Length: 456
• Form: Lyophilized
• Molecular Mass: 51.5 kDa
• N-terminal Sequence Analysis: Phe 19
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > (17.0+77.6) % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD274 CD274 molecule [ Canis lupus familiaris ]
• Official Symbol: CD274
• Synonyms: PDL1; PD-L1; programmed death ligand 1
• Gene ID: 484186
• mRNA Refseq: NM_001291972
• Protein Refseq: NP_001278901