Recombinant Canine CD47 protein, His-tagged

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Recombinant Canine CD47 protein, His-tagged

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Cat.No. : CD47-862C
Product Overview : Recombinant Canine CD47 (Gln19-Asn140) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
Source : HEK293
Species : Canine
Tag : His
Predicted N Terminal : Gln19
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 15.8 kDa. The protein migrates as 27-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln19-Asn140
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by reduced SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD47-862C :

Canine CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of CD47-862C :

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) at 5 μg/mL (100 μL/well) can bind Canine CD47, His Tag with a linear range of 5-78 ng/mL (QC tested).

Gene Name : CD47
Official Symbol : CD47
Synonyms : CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer) , MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6
Gene ID : 478552
mRNA Refseq : NM_001080721.1
Protein Refseq : NP_001074190.1
UniProt ID : J9P735

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