Recombinant Human CD47, Fc tagged

• Cat.No.: CD47-7889H
• Product Overview: Recombinant Human CD47 (NP_942088.1) (Met1-Pro139), fused with the Fc region of human IgG1 at the C-terminus, was produced in Human Cells.

Specification

• Source: Human Cells
• Species: Human
• Tag: Non
• Predicted N Terminal: Gln 19
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CD47/Fc is a disulfide-linked homodimer. The reduced monomer comprises 362 amino acids and has a predicted molecular mass of 40.7 kDa. The apparent molecular mass of the protein is approximately 48-60 kDa in SDS-PAGE under reducing c
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: Met1-Pro139

Gene Information

• Gene Name: CD47 CD47 molecule [ Homo sapiens ]
• Official Symbol: CD47
• Gene ID: 961
• mRNA Refseq: NM_001777
• Protein Refseq: NP_001768
• MIM: 601028
• UniProt ID: Q08722
• Chromosome Location: 3q13.1-q13.2

Citation

Co-coating of receptor-targeted drug nanocarriers with anti-phagocytic moieties enhances specific tissue uptake versus non-specific phagocytic clearance

Journal: Biomaterials    PubMed ID: 28923682    Data: 2018/12/1

Authors: Joshua Kim, Saraudeep Sinha, Silvia Muro

Article Snippet:Monoclonal antibodies recognizing human or murine intercellular adhesion molecule 1 (ICAM-1), a cell-surface receptor overexpressed in many diseases, 35 were from hybridomas from American Type Culture Collection (Manassas, VA).Monoclonal antibodies recognizing human or murine intercellular adhesion molecule 1 (ICAM-1), a cell-surface receptor overexpressed in many diseases, 35 were from hybridomas from American Type Culture Collection (Manassas, VA).. Recombinant human or murine CD47, tagged with poly-histidine or human Fc, were from LD Biopharma Inc. (San Diego, CA) and Creative BioMart (Shirley, NY), respectively.. Antibodies to thrombospondin-1 (TSP-1) and integrin α v β 3 were from ThermoFisher Scientific (Waltham, MA) and R&D systems (Minneapolis, MN) respectively.Antibodies to thrombospondin-1 (TSP-1) and integrin α v β 3 were from ThermoFisher Scientific (Waltham, MA) and R&D systems (Minneapolis, MN) respectively.

Publication :

Co-coating of receptor-targeted drug nanocarriers with anti-phagocytic moieties enhances specific tissue uptake versus non-specific phagocytic clearance (2017)