Recombinant Canine EGF Protein, His-tagged

Cat.No.: EGF-362C
Product Overview: Recombinant Canine EGF (Q9BEA0) (Asn 973-Arg 1024) was fused with a polyhistidine tag at the N-terminus.
Description: EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of bi centigradehemical changes within the cell- a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR- that ultimately lead to DNA synthesis and cell proliferation.
Source: E. coli
Species: Canine
Tag : His
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.5. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity: Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts. The EC50 for this effect is typically 0.1-0.5 ng/ml.
Molecular Mass: The recombinant canine EGF consists of 68 amino acids and has a calculated molecular mass of 8.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of canine EGF is approximately 12 kDa.
Protein length: Asn 973-Arg 1024
Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
Purity: > 98 % as determined by SDS-PAGE
Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Official Symbol: EGF
Synonyms: EGF;CEGF;pro-epidermal growth factor;epidermal growth factor (beta-urogastrone)

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