Active Recombinant Mouse Egf Protein (Asn977-Arg1029), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Egf-21M
• Product Overview: Recombinant Mouse Egf Protein (Asn977-Arg1029) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Epidermal Growth Factors (EGF) is a member of the EGF-family of proteins. EGF is mainly secreted from ectodermal cells, monocytes, kidney and duodenal glands. Upon binding to its receptor, EGFR, EGF acts to stimulate cell growth and proliferation of epithelial cells, play important roles in many developmental processes including accelerate tooth eruption, inhibits gastric acid secretion, and involve in wound healing.
• Source: E. coli
• Species: Mouse
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 80 pg/mL. The specific activity of recombinant mouse EGF is approximately >1 × 10⁷ IU/mg.
• Molecular Mass: The protein has a calculated MW of 6.98 kDa.
  The protein migrates as 8 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Asn977-Arg1029
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Egf epidermal growth factor [ Mus musculus (house mouse) ]
• Official Symbol: Egf
• Synonyms: EGF; epidermal growth factor; pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); AI790464
• Gene ID: 13645
• mRNA Refseq: NM_010113
• Protein Refseq: NP_034243
• UniProt ID: P01132