Description : |
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. |
Source : |
E.coli |
Species : |
Canine |
Form : |
Lyophilized from a 0.2 µm filtered concentrated solution in 25 mM Sodium Succinate, pH 5.0, 60 mM NaCl, with 0.1 % Tween-80. |
Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using A-72 canine fibroma cells infected with vesicular stomatitis virus (VSV) is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg. |
Molecular Mass : |
Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 143 amino acids. |
Protein length : |
143 |
AA Sequence : |
QAMFFKEIENLKEYFNASNPDVSDG GSLFVDILKKWREESDKTIIQSQIV SFYLKLFDNFKDNQIIQRSMDTIKE DMLGKFLNSSTSKREDFLKLIQIPV NDLQVQRKAINELIKVMNDLSPRSN LRKRKRSQNLFRGRRASK |
Endotoxin : |
Less than 0.1 EU/µg of rCaIFN-γ as determined by LAL method. |
Purity : |
>97% by SDS-PAGE and HPLC analyses. |
Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |