Recombinant Canine IL3RA cell lysate
|Product Overview :||Canine IL3RA derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls|
- Gene Information
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|Source :||Human cells|
|Preparation method :||Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.|
|Lysis buffer :||Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Quality control Testing :||12.5% SDS-PAGE Stained with Coomassie Blue|
|Recommended Usage :||1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.|
|Stability :||Samples are stable for up to twelve months from date of receipt at -80°C|
|Storage Buffer :||50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Storage Instruction :||Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.|
|Gene Name :||IL3RA interleukin 3 receptor, alpha (low affinity) [ Canis lupus familiaris ]|
|Official Symbol :||IL3RA|
|Synonyms :||interleukin-3 receptor subunit alpha; interleukin 3 receptor, alpha (low affinity)|
|Gene ID :||609293|
|mRNA Refseq :||XM_005641060|
|Protein Refseq :||XP_005641117|
|Chromosome Location :||chromosome: X|
|Pathway :||Apoptosis, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (6)Ask a question
The feasibility of personalized therapy depends on the specific disease type and patient situation, and more research is needed to determine the application of personalized therapy to Il3ra.
Il3ra has certain promise in clinical application, especially in the treatment of immune-related diseases.
Regulates the proliferation and differentiation of immune cells by binding to its interacting cytokine IL-3, and plays a role in regulating the function of the immune system.
Clinical trials of recombinant Il3ra have been conducted in different disease areas. At present, some early trials show its potential in the treatment of certain diseases, but more research is needed to verify its efficacy and safety.
The Il3ra can regulate the proliferation and differentiation of different types of immune cells in the immune system, thus affecting the function of the immune system.
Il3ra is generally well tolerated, but some minor side effects such as headache and nausea may occur. Serious side effects are rare.
Customer Reviews (3)Write a review
In special environment, good stability. -
Good crystallization property and good effect. -
Has the expected biological activity, such as enzyme activity and receptor binding activity, and so on. -
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