Recombinant Canine TNFRSF18 protein, His-tagged
|Product Overview :||Recombinant Canine TNFRSF18 (Gly23-Pro154) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.|
|Predicted N Terminal :||Gly23|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 15.8 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Gly23-Pro154|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of TNFRSF18-2511C :||
Canine GITR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of TNFRSF18-2511C :||
Immobilized Canine GITR, His Tag at 2 μg/mL (100 μL/well) can bind Human GITR Ligand, Fc Tag with a linear range of 2-40 ng/mL (QC tested).
|Gene Name :||TNFRSF18|
|Official Symbol :||TNFRSF18|
|Gene ID :||606971|
|mRNA Refseq :||NM_001190744.1|
|Protein Refseq :||NP_001177673.1|
|UniProt ID :||D7F619|
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