Recombinant Canine TNFRSF18 protein, His-tagged

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Recombinant Canine TNFRSF18 protein, His-tagged

TNFRSF18 Related Products

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Cat.No. : TNFRSF18-2511C
Product Overview : Recombinant Canine TNFRSF18 (Gly23-Pro154) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Source : HEK293
Species : Canine
Tag : His
Predicted N Terminal : Gly23
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 15.8 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gly23-Pro154
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of TNFRSF18-2511C :

Canine GITR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of TNFRSF18-2511C :

Immobilized Canine GITR, His Tag at 2 μg/mL (100 μL/well) can bind Human GITR Ligand, Fc Tag with a linear range of 2-40 ng/mL (QC tested).

Gene Name : TNFRSF18
Official Symbol : TNFRSF18
Synonyms : GITR
Gene ID : 606971
mRNA Refseq : NM_001190744.1
Protein Refseq : NP_001177673.1
UniProt ID : D7F619

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