Recombinant Chicken IL4 protein, His & T7-tagged

• Cat.No.: IL4-645C
• Product Overview: Recombinant Chicken IL4 aa. (Leu26~Lys136 (Accession# Q5W4U1)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Chicken
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.2kDa
• Protein length: Leu26~Lys136 (Accession# Q5W4U1)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: IL4 interleukin 4 [ Gallus gallus (chicken) ]
• Official Symbol: IL4
• Synonyms: interleukin-4
• Gene ID: 416330
• mRNA Refseq: NM_001007079.1
• Protein Refseq: NP_001007080.1
• UniProt ID: Q5W4U1

Reviews

10/17/2021

By testing immunogenicity in animal models, detect whether an immune response is induced.

11/09/2020

The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

05/06/2020

This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.