Recombinant Chicken Interleukin 2

• Cat.No.: IL2-249C
• Product Overview: Recombinant Chicken IL-2 was expressed inYeast. MW= 14.0 kDa (121 aa).

Specification

• Species: Chicken
• Source: Yeast
• Tag: Non
• Description: Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body"s natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity.
• Formulation/ Reconstitution: Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Purification: Ion-exchange chromatography.
• Purity: >95% as visualized by SDS-PAGE analysis.
• Amino Acid Sequence: ASLSSAKRKP LQTLIKDLEI LENIKNKIHL ELYTPTETQE CTQQTLQCYL GEVVTLKKET EDDTEIKEEF VTAIQNIEKN LKSLTGLNHT GSECKICEAN NKKKFPDFLH ELTNFVRYLQ K.
• Bioactivity: In testing.
• Stability And Storage: Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL2 interleukin 2 [ Gallus gallus ]
• Synonyms: interleukin 2; IL2; IL-2; interleukin-2
• Gene ID: 373958
• mRNA Refseq: NM_204153.1
• Protein Refseq: NP_989484.1
• Chromosome Location: 4

Citation

Clonal anergy of CD117 + chB6 + B cell progenitors induced by avian leukosis virus subgroup J is associated with immunological tolerance

Journal: Retrovirology    PubMed ID: 30602379    Data: 2019/1/3

Authors: Shuhai He, Gaoying Zheng, Ziqiang Cheng

Article Snippet:The purity of the sorted cells was ≥ 98%, as analysed with FACSDiva software (BD Biosciences).The purity of the sorted cells was ≥ 98%, as analysed with FACSDiva software (BD Biosciences).. Then, these CD117 + chB6 + cells were seeded in 24-well plates at 1 × 10 6 cells/mL and maintained at 37 °C with 5% CO 2 in a culture medium containing IMDM (Gibco, California, USA), 20% chicken serum (Gibco, California, USA), 50 ng/mL chicken stem cell growth factor (SCF) and 20 ng/mL chicken interleukin-7 (IL-7) (Creative BioMart, New York, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin.