Recombinant CMV gB protein

• Cat.No.: gB-0297C
• Product Overview: Recombinant CMV gB protein(Met 1-Lys 700&Arg 777-Val 907)(AAA45920.1,with furin cleavage site mutated from 'RTKR' to 'TTQT') was expressed in HEK293, fused with five additional amino acids (DDDDK) at the C-terminus.

Specification

• Species: CMV
• Source: HEK293
• Tag: Non
• Protein Length: 1-700 aa&777-907 aa
• Form: Lyophilized from sterile Tris 50mM, NaCl 100mM, pH 8.0.
• Molecular Mass: The recombinant glycoprotein B of human CMV consists of 813 amino acids and has a predicted molecular mass of 92 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB is approximately 130-140 kDa due to glycosylation.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: gB