Active Recombinant CMV gB protein, hFc-tagged

• Cat.No.: gB-109C
• Product Overview: Active Recombinant CMV gB protein(Met 1-Lys 700)(AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') precursor was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: CMV
• Source: HEK293
• Tag: Fc
• Protein Length: 1-700 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to bind biotinylated Human CD209-Fc in functional ELISA.
• Molecular Mass: The recombinant human CMV glycoprotein B ECD/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 914 amino acids and has a predicted molecular mass of 104 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the monomer is approximately 135-145 kDa due to glycosylation
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: gB