Recombinant COVID-19 Spike protein, His-tagged

• Cat.No.: Spike-3432V
• Product Overview: Recombinant COVID-19 Spike protein(P0DTC2)(319-541aa(L452Q,F490S)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: C-His
• Protein length: 319-541aa(L452Q,F490S)
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 27.8 kDa
• AASequence: RVQPTESIVRFPNITNLCPFGEVFN ATRFASVYAWNRKRISNCVADYSVL YNSASFSTFKCYGVSPTKLNDLCFT NVYADSFVIRGDEVRQIAPGQTGKI ADYNYKLPDDFTGCVIAWNSNNLDS KVGGNYNYQYRLFRKSNLKPFERDI STEIYQAGSTPCNGVEGFNCYSPLQ SYGFQPTNGVGYQPYRVVVLSFELL HAPATVCGPKKSTNLVKNKCVNF
• Purity: Greater than 90% as determined by SDS-PAGE.
  Greater than 90% as determined by SEC-HPLC.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.