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Recombinant Cynomolgus ANGPT1 Protein (Arg277-Phe498), HlgG1 Fc-tagged

Cat.No. : ANGPT1-1066C
Product Overview : Recombinant Cynomolgus ANGPT1 Protein (XP_005563980.1) (Arg277-Phe498) was produced by HEK293 Cells expression system. This protein was expressed with the Fc region of human IgG1 at the N-terminus.
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Description : The angiopoietin (ANGPT)-TIE2/TEK signaling pathway is essential for blood and lymphatic vascular homeostasis. ANGPT1 is a potent TIE2 activator, whereas ANGPT2 functions as a context-dependent agonist/antagonist. In disease, ANGPT2-mediated inhibition of TIE2 in blood vessels is linked to vascular leak, inflammation, and metastasis. Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemm's canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. A role for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By linking ANGPT1 with PCG, these results highlight the importance of ANGPT/TEK signaling in glaucoma pathogenesis and identify a candidate target for therapeutic development.
Source : HEK293
Species : Cynomolgus
Tag : HlgG1 Fc
Predicted N Terminal : Glu
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant cynomolgus ANGPT1 consists of 482 amino acids and predicts a molecular mass of 54.1 kDa.
Protein length : Arg277-Phe498
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 90 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : ANGPT1 angiopoietin 1 [ Macaca fascicularis (crab-eating macaque) ]
Official Symbol : ANGPT1
Gene ID : 102123994
mRNA Refseq : XM_005563923
Protein Refseq : XP_005563980

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (18)

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Is there any research on the role of ANGPT1 in neurological disorders? 12/04/2022

While ANGPT1 is primarily associated with blood vessel formation and stability, recent studies have indicated potential involvement of ANGPT1 in neurological disorders. For example, it has been suggested that ANGPT1 may play a role in Alzheimer's disease, stroke, and neuroinflammation. However, further research is required to understand the precise role of ANGPT1 in these neurological conditions.

Are there any known interactions or regulators of ANGPT1? 09/14/2022

The activity of ANGPT1 is tightly regulated by different factors. Vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta) are known to collaborate with ANGPT1 to regulate blood vessel formation and stability. Several transcription factors, signaling molecules, and microRNAs have also been identified as regulators of ANGPT1 expression.

Does ANGPT1 play a role in vascular development during embryogenesis? 12/29/2021

Yes, ANGPT1 is involved in vascular development during embryogenesis. It promotes blood vessel maturation and stabilization, which is essential for the formation of a functional vascular system. ANGPT1, along with its receptor Tie2, regulates the interactions between endothelial cells and pericytes, leading to the formation of stable blood vessels. Deficiency in ANGPT1 or Tie2 during embryonic development can result in abnormal blood vessel formation and defects in vascular morphogenesis.

Can ANGPT1 be used as a therapeutic target for cancer? 08/05/2021

ANGPT1 has been implicated in tumor angiogenesis, which is the process of formation of new blood vessels to supply oxygen and nutrients to tumors. Its expression is often elevated in certain types of cancer, and it promotes tumor growth and metastasis. Therefore, ANGPT1 has been considered as a potential therapeutic target for cancer. Several approaches, such as blocking ANGPT1/Tie2 signaling using antibodies or small molecules, have shown promising results in preclinical studies. However, further research is needed to determine the efficacy and safety of ANGPT1-based therapies in clinical settings.

Are there any known inhibitors or activators of ANGPT1? 07/13/2021

Several factors have been identified that can either inhibit or activate ANGPT1 signaling. For example, VEGF, another pro-angiogenic factor, can promote ANGPT1 expression and enhance its activity. On the other hand, certain molecules, such as angiostatin and endostatin, have been shown to block ANGPT1-induced angiogenesis. Furthermore, there are ongoing studies to develop therapeutics that directly target ANGPT1 or its receptor Tie2 to modulate its activity.

Is ANGPT1 involved in wound healing? 03/04/2021

Yes, ANGPT1 is involved in the process of wound healing. It promotes angiogenesis in the injured area, which helps to supply oxygen and nutrients to the healing tissues. ANGPT1 also plays a role in tissue remodeling and repair by promoting the migration of certain cells, such as fibroblasts, which contribute to wound closure. Additionally, it may help to reduce inflammation and support the formation of new blood vessels during the healing process.

Are there any ongoing clinical trials investigating ANGPT1 in the context of cancer or other diseases? 01/14/2021

While there are currently no registered clinical trials specifically investigating ANGPT1 as a therapeutic target, there are ongoing studies exploring the efficacy of agents that block or modulate ANGPT1 signaling in various diseases, including cancer. These studies aim to evaluate the safety and effectiveness of ANGPT1-targeted therapies in preclinical and clinical settings and may provide valuable insights into the potential clinical applications of ANGPT1 inhibition or stimulation.

Is ANGPT1 involved in wound healing? 11/12/2020

Yes, ANGPT1 has been implicated in wound healing and tissue repair processes. It promotes angiogenesis and stabilizes newly formed blood vessels, which are crucial for delivering oxygen and nutrients to healing tissues. ANGPT1 also stimulates the recruitment and maturation of pericytes, which aid in wound healing by providing structural support to blood vessels.

Is ANGPT1 associated with lymphangiogenesis? 06/13/2020

While ANGPT1's primary role is in angiogenesis, the formation of blood vessels, it has also been reported to play a role in lymphangiogenesis, the formation of lymphatic vessels. ANGPT1-Tie2 signaling has been shown to regulate lymphatic vessel growth and maturation. However, the precise mechanisms and significance of ANGPT1 in lymphangiogenesis are still under investigation.

Are there any genetic disorders associated with ANGPT1? 05/16/2020

Currently, no inherited disorders directly linked to ANGPT1 gene mutations have been reported. However, dysregulation of ANGPT1 expression and abnormal Tie2 signaling have been implicated in various diseases, including retinopathy, inflammatory conditions, and cancer.

Are there any clinical trials underway investigating ANGPT1-based therapies? 05/15/2020

At present, there are no clinical trials specifically focused on ANGPT1-based therapies. However, there are clinical trials investigating the use of ANGPT1 in combination with other molecules, such as VEGF inhibitors or immune checkpoint inhibitors, for various cancers. These trials aim to assess the efficacy and safety of ANGPT1-targeted therapies as part of a comprehensive treatment strategy.

Are there any genetic mutations or polymorphisms in the ANGPT1 gene associated with diseases? 04/16/2019

While genetic mutations or polymorphisms in the ANGPT1 gene have not been extensively studied, some studies have reported associations with certain diseases. For example, specific variations in the ANGPT1 gene have been linked to susceptibility to age-related macular degeneration (AMD) and diabetic retinopathy. However, more research is needed to establish the significance of these genetic variants and their impact on disease development.

Can ANGPT1 be used as a diagnostic marker for any diseases? 11/18/2018

ANGPT1 is not commonly used as a diagnostic marker for specific diseases. However, its levels have been found to be altered in certain conditions, such as cardiovascular diseases, diabetic retinopathy, and sepsis, which suggests its potential as a biomarker. Further research is needed to establish ANGPT1 as a reliable diagnostic marker.

How is ANGPT1 regulated at the transcriptional level? 10/03/2018

The transcriptional regulation of ANGPT1 is complex and involves various factors. Several transcription factors, such as hypoxia-inducible factors (HIF-1α), Sp1, and NF-κB, have been shown to directly bind to the ANGPT1 gene promoter and regulate its expression. In addition, signaling pathways like VEGF and TGF-beta can influence ANGPT1 expression by activating downstream transcription factors that interact with the ANGPT1 promoter region. MicroRNAs also play a role in post-transcriptional regulation of ANGPT1 expression.

What are the potential therapeutic applications of ANGPT1? 03/18/2018

ANGPT1 has shown potential therapeutic value in various medical conditions. It is being explored for its ability to promote blood vessel formation and stabilize existing blood vessels in disorders such as cardiovascular diseases, diabetic retinopathy, and tumor angiogenesis. Additionally, ANGPT1 has been investigated for its potential in tissue engineering and regenerative medicine approaches.

What are the potential side effects or concerns associated with targeting ANGPT1 for therapy? 05/21/2017

One potential concern with targeting ANGPT1 for therapy is the potential for adverse effects on normal physiological processes. ANGPT1 plays a crucial role in vascular development and stability, and its blockade or manipulation could have unforeseen consequences on blood vessel formation and function. Additionally, since ANGPT1 is involved in various biological processes, there might be off-target effects that could lead to unintended consequences. Therefore, careful evaluation of ANGPT1-targeted therapies is necessary to ensure safety and minimize potential side effects.

Is ANGPT1 involved in cancer progression or metastasis? 11/04/2016

ANGPT1 can have both tumor-suppressive and tumor-promoting effects depending on the context. In some cancers, ANGPT1/Tie2 signaling can inhibit tumor angiogenesis and limit metastasis. Conversely, in certain types of tumors, ANGPT1 can induce angiogenesis, promoting tumor growth and metastasis. Further research is necessary to understand the complexities of ANGPT1's role in different types and stages of cancer.

Are there any inhibitors or activators of ANGPT1 that have been identified? 09/18/2016

Several molecules have been identified as modulators of ANGPT1/Tie2 signaling. For example, antibodies that activate or block Tie2 receptor have been developed for experimental purposes. In addition, small-molecule inhibitors targeting the ANGPT1/Tie2 pathway are being investigated as potential therapeutic agents for diseases involving abnormal angiogenesis.

Customer Reviews (5)

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Reviews
02/19/2022

    ANGPT1 protein's utility extends to protein electron microscopy structure analysis, where it plays a crucial role in investigating the detailed architecture and conformational changes of proteins.

    09/10/2020

      It exhibits exceptional performance in ELISA assays, making it an excellent choice for researchers studying angiopoietins, angiogenesis, or vascular biology.

      08/24/2020

        It demonstrates excellent performance in ELISA assays, making it an ideal choice for researchers studying protein-protein interactions, antibody detection, or biomarker identification.

        08/21/2019

          ANGPT1 protein is highly recommended for use in various research applications, including ELISA and protein electron microscopy structure analysis.

          10/14/2018

            ANGPT1 protein's remarkable performance in ELISA and protein electron microscopy structure analysis positions it as a valuable tool in various fields, including cardiovascular research, developmental biology, and molecular medicine.

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