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Recombinant Cynomolgus CD200R1L cell lysate

Cat.No. : CD200R1L-2148CCL
Product Overview : Cynomolgus CD200Rla derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Cynomolgus
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CD200R1L CD200 receptor 1-like [ Macaca fascicularis (crab-eating macaque) ]
Official Symbol : CD200R1L
Synonyms : CD200 receptor 1-like
Gene ID : 102140606
mRNA Refseq : XM_005548154
Protein Refseq : XP_005548211
Chromosome Location : chromosome: 2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How does the expression level of CD200R1L protein change during the development of the disease? 06/20/2022

The expression level of CD200R1L proteins may change during the development and progression of certain diseases. For example, in some immune diseases, the expression level of CD200R1L may be down-regulated, leading to increased immune cell activity and an intensified inflammatory response. However, the association between specific changes and different diseases and the regulatory mechanism need further research to clarify.

What are the methods of up-regulating and down-regulating CD200R1L protein? 09/06/2021

Up-regulation and down-regulation of CD200R1L proteins can be achieved through a variety of pathways. For example, specific agonists or inhibitors can be used to modulate the signaling pathways of CD200R1L, affecting their expression and function. In addition, certain cytokines and extracellular signaling molecules may also play regulatory roles by influencing the expression of CD200R1L. Specific up-regulation and down-regulation methods need to select appropriate strategies according to the research purpose and experimental conditions.

Is there a link between gene polymorphisms in CD200R1L proteins and susceptibility to disease? 05/08/2021

Polymorphisms in CD200R1L genes may be associated with susceptibility to certain diseases, but this is currently not well studied. Some studies suggest that polymorphismic variations in CD200R1L genes may affect the expression level, function, and interaction with the ligand CD200, thereby affecting immunomodulatory processes. However, more research is needed to verify this association.

What is the relationship between CD200R1L protein and tumor immune escape? 02/03/2021

CD200R1L proteins play a role in tumor immune evasion. Studies have found that some tumor cells can inhibit the activity of CD200R1L-positive immune cells (such as macrophages, dendritic cells, etc.) by expressing CD200 ligands highly, reducing the killing effect of immune cells on tumor cells, thereby promoting tumor growth and metastasis. The regulation of CD200R1L signaling pathways may help reverse tumor immune escape and improve the effectiveness of tumor immunotherapy.

How does CD200R1L protein play a role in immune tolerance? 01/13/2020

It play a role in inhibiting the activity of immune cells in immune tolerance. After binding to its ligand CD200, CD200R1L reduces the production of pro-inflammatory cytokines by inhibiting the activation signaling pathway of immune cells, thereby regulating the intensity of the immune response, maintaining the balance of the immune system, and preventing tissue damage caused by excessive immune response.

What is the potential application value of CD200R1L protein in immunotherapy? 07/04/2019

CD200R1L proteins have potential applications in immunotherapy. Studies have shown that by regulating CD200R1L signaling pathways, immune cells can enhance the ability of immune cells to attack tumor cells and improve the effect of immunotherapy. In addition, the regulatory role of CD200R1L in autoimmune diseases may also provide new ideas for the treatment of related diseases.

Customer Reviews (3)

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10/27/2022

    The wide range of applications of CD200R1L shows its important role in scientific research and provides strong support for scientific research.

    07/06/2021

      After using CD200R1L, the background of the Western blot was very clean with no nonspecific signal.

      01/08/2020

        This stability of CD200R1L was still very good in the process of performing complex biological experiments, indicating that it has excellent within-batch and between-batch stability.

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