| Source : |
HEK293 |
| Species : |
Cynomolgus |
| Tag : |
C-hFc |
| Protein length : |
Met1-Pro152 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
1. Immobilized Recombinant Human CD86 / B7-2 Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus CD28 / TP44 Protein (Fc Tag, ECD), the EC50 is 100-420 ng/mL. 2. Immobilized Recombinant Human CD80 / B7-1 Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus CD28 / TP44 Protein (Fc Tag, ECD), the EC50 is 600-1400 ng/mL. |
| Molecular Mass : |
The recombinant cynomolgus CD28 consists 372 amino acids and predicts a molecular mass of 41.9 kDa. |
| Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
| Purity : |
> 90 % as determined by SDS-PAGE. |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
