Recombinant Cynomolgus CD8A & CD8B Protein, Flag & His-tagged
Cat.No. : | CD8A&CD8B-948C |
Product Overview : | Recombinant Cynomolgus CD8A(XP_001092778.2) (Met42-Asp223) was fused with a flag tag at the C-terminus, constructed the plasmid 1 ; Recombinant Cynomolgus CD8B (XP_005575417.1) (Met1-Pro170) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. |
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Source : | HEK293 |
Species : | Cynomolgus |
Tag : | Flag & His |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Molecular Mass : | The recombinant cynomolgus CD8A & CD8B heterodimer comprises 395 (202+193) amino acids and has a calculated molecular mass of 44.27 (22.51+21.76) kDa. It migrates as an approximately 33.52 KDa band in SDS-PAGE under reducing conditions. |
Protein length : | Met42-Asp223 & Met1-Pro170 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C. |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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