Recombinant Cynomolgus CTLA4 protein, Avi & His-tagged, Biotinylated

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Recombinant Cynomolgus CTLA4 protein, Avi & His-tagged, Biotinylated

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Cat.No. : CTLA4-354C
Product Overview : Biotinylated Cynomolgus CTLA-4 is expressed from human HEK293 cells. It contains AA Ala 37 - Ser 160 (Accession # G7PL88). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Description : CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Source : HEK293
Species : Cynomolgus
Tag : Avi & His
Predicted N Terminal : Ala 37
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Human B7-1, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CTLA-4, Avi Tag, His Tag with a linear range of 2-31 ng/mL. Immobilized Human B7-2, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CTLA-4, Avi Tag, His Tag with a linear range of 2-63 ng/mL.
Molecular Mass : This protein carries a Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 15.9 kDa. The protein migrates as 23-26 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Protein length : Ala 37 - Ser 160
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Storage buffer : PBS, pH 7.4
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : Powder,RT
SDS-PAGE :

Biotinylated Cynomolgus CTLA-4 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Gene Name : CTLA4 cytotoxic T-lymphocyte associated protein 4 [ Macaca fascicularis (crab-eating macaque) ]
Official Symbol : CTLA4
Synonyms : cytotoxic T-lymphocyte protein 4
Gene ID : 102115124
mRNA Refseq : XM_005574014.2
Protein Refseq : XP_005574071.1
UniProt ID : G7PL88

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