Active Recombinant Cynomolgus CYP2C76 Protein

• Cat.No.: CYP2C76-127C
• Product Overview: Recombinant cynomolgus CYP2C76 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: Cynomolgus CYP2C76, an important drug-metabolizing enzyme, accounts for drug metabolism differences to humans, so that CYP2C76-null animals might show drug-metabolizing properties more similar to humans.
• Source: E. coli
• Species: Cynomolgus
• Bio-activity: Cytochrome c Reductase Activity: 321 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Tolbutamide 4-hydroxylase
  Linear with time up to: 25 min(Determined at 350 µM substrate, using a P450 concentration of 125 pmol/ml)
  Linear with P450 up to: 200 pmol/ml(Determined at 350 µM substrate, using an incubation time of 20 min)
  Vmax: 1.3 pmol/min/pmol CYP
  Km: 624 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 8.8 nmol/ml
  Protein Concentration: 10.9 mg/ml
  Specific P450 Content: 807 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2C76 cytochrome P450 family 2 subfamily C member 76 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: CYP2C76
• Synonyms: CYP2C76; cytochrome P450 family 2 subfamily C member 76; cytochrome P450 family 2 subfamily C member 76; cytochrome P450 2C76
• Gene ID: 102114994
• mRNA Refseq: NM_001284789
• Protein Refseq: NP_001271718
• UniProt ID: Q0HA17