Active Recombinant Cynomolgus CYP2C8 Protein

• Cat.No.: CYP2C8-124C
• Product Overview: Recombinant cynomolgus CYP2C8 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
• Source: E. coli
• Species: Cynomolgus
• Bio-activity: Cytochrome c Reductase Activity: 145 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Paclitaxel 6α-hydroxylase
  Linear with time up to: 20 min(Determined at 15 µM substrate, using a P450 concentration of 125 pmol/ml)
  Linear with P450 up to: 100 pmol/ml(Determined at 15 µM substrate, using an incubation time of 20 min)
  Vmax: 0.14 pmol/min/pmol CYP
  Km: 18 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 3.1 nmol/ml
  Protein Concentration: 8.3 mg/ml
  Specific P450 Content: 373 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2C8 cytochrome P450 family 2 subfamily C member 8 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: CYP2C8
• Synonyms: CYP2C8; cytochrome P450 family 2 subfamily C member 8; CYP2C20; CYPIIC20; cytochrome P450 2C20; Cytochrome P450 2C20; Cytochrome P450-MK1; Cytochrome P450-MKMP13; P-450 2C; cytochrome P-450 2C
• Gene ID: 102117212
• mRNA Refseq: NM_001283763
• Protein Refseq: NP_001270692
• UniProt ID: P33262