| Cat.No. : |
CYP2C8-124C |
| Product Overview : |
Recombinant cynomolgus CYP2C8 protein and human CYP-reductase without tag were coexpressed in E. coli. |
| Description : |
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. |
| Source : |
E. coli |
| Species : |
Cynomolgus |
| Bio-activity : |
Cytochrome c Reductase Activity: 145 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Paclitaxel 6α-hydroxylase
Linear with time up to: 20 min(Determined at 15 µM substrate, using a P450 concentration of 125 pmol/ml)
Linear with P450 up to: 100 pmol/ml(Determined at 15 µM substrate, using an incubation time of 20 min)
Vmax: 0.14 pmol/min/pmol CYP
Km: 18 μM |
| Usage : |
For laboratory (research) purposes only |
| Storage : |
Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
| Concentration : |
P450 Concentration: 3.1 nmol/ml
Protein Concentration: 8.3 mg/ml
Specific P450 Content: 373 pmol/mg protein |
| Storage Buffer : |
50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.