Recombinant Cynomolgus CYP2C8 Protein

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Recombinant Cynomolgus CYP2C8 Protein

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Cat.No. : CYP2C8-124C
Product Overview : Recombinant cynomolgus CYP2C8 protein and human CYP-reductase without tag were coexpressed in E. coli.
Description : Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Source : E. coli
Species : Cynomolgus
Bio-activity : Cytochrome c Reductase Activity: 145 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Paclitaxel 6α-hydroxylase
Linear with time up to: 20 min(Determined at 15 µM substrate, using a P450 concentration of 125 pmol/ml)
Linear with P450 up to: 100 pmol/ml(Determined at 15 µM substrate, using an incubation time of 20 min)
Vmax: 0.14 pmol/min/pmol CYP
Km: 18 μM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 3.1 nmol/ml
Protein Concentration: 8.3 mg/ml
Specific P450 Content: 373 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CYP2C8 cytochrome P450 family 2 subfamily C member 8 [ Macaca fascicularis (crab-eating macaque) ]
Official Symbol : CYP2C8
Synonyms : CYP2C8; cytochrome P450 family 2 subfamily C member 8; CYP2C20; CYPIIC20; cytochrome P450 2C20; Cytochrome P450 2C20; Cytochrome P450-MK1; Cytochrome P450-MKMP13; P-450 2C; cytochrome P-450 2C
Gene ID : 102117212
mRNA Refseq : NM_001283763
Protein Refseq : NP_001270692
UniProt ID : P33262

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