Recombinant Cynomolgus CYP2C9 Protein


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Recombinant Cynomolgus CYP2C9 Protein

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Cat.No. : CYP2C9-125C
Product Overview : Recombinant cynomolgus CYP2C9 protein and human CYP-reductase without tag were coexpressed in E. coli.
Description : CYP2C9 mediated oxidation of valproate, which is the minor metabolic pathway in adults, appears to become the principal route in children. Genetic and non-genetic variations in CYP2C9 activity can result in significant inter- and intra-individual differences in valproate pharmacokinetics and valproate induced adverse reactions.
Source : E. coli
Species : Cynomolgus
Bio-activity : Cytochrome c Reductase Activity: 191 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Testosterone 17-oxidase
Linear with time up to: 30 min(Determined at 15 µM substrate, using a P450 concentration of 10 pmol/ml)
Linear with P450 up to: 50 pmol/ml(Determined at 15 µM substrate, using an incubation time of 5 min)
Vmax: 15 pmol/min/pmol CYP
Km: 15 μM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 2.8 nmol/ml
Protein Concentration: 9.4 mg/ml
Specific P450 Content: 298 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CYP2C9 cytochrome P450 family 2 subfamily C member 9 [ Macaca fascicularis (crab-eating macaque) ]
Official Symbol : CYP2C9
Synonyms : CYP2C9; cytochrome P450 family 2 subfamily C member 9; CYP2C43; cytochrome P450 2C9-like; cytochrome P450 family 2 subfamily C member 9; cytochrome P450 2C43; cytochrome P450, family 2, subfamily C, polypeptide 9
Gene ID : 102115450
mRNA Refseq : NM_001287625
Protein Refseq : NP_001274554
UniProt ID : Q0HA18

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