Recombinant Cynomolgus FAP Protein, His-tagged
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Cat.No. : | FAP-370C Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant Cynomolgus FAP (XP_005573377.1) (Arg30-Asp760) was expressed with a polyhistidine tag at the N-terminus. |
Description : | Seprase, also known as 170 kDa melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to the peptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 170-kDa dimer, consisting of two 97-kDa subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV / CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP col centigradealized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP col centigradealized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-ass centigradeiated molecules. In ass centigradeiation with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. |
Source : | HEK293 |
Species : | Cynomolgus |
Tag : | His |
Predicted N Terminal : | His |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its ability to convert the substrate benzyloxycarbonylGlyPro7amido 4methylcoumarin (ZGPAMC) to ZGlyPro and 7amino4methylcoumarin (AMC). The specific activity is >600 pmol/min/µg. |
Molecular Mass : | The recombinant FAP consists of 750 amino acids and predicts a molecular mass of 87 kDa. |
Protein length : | Arg30-Asp760 |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 90 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
Official Symbol : | FAP |
Synonyms : | FAP;fibroblast activation protein alpha;prolyl endopeptidase FAP |
◆ Recombinant Protein
FAP-715H | Recombinant Human FAP Protein, His-tagged | +Inquiry |
FAP-006H | Recombinant Human FAP Protein, His-tagged | +Inquiry |
FAP-371H | Recombinant Human FAP Protein, His-tagged | +Inquiry |
Fap-1018M | Recombinant Mouse Fap Protein, MYC/DDK-tagged | +Inquiry |
FAP-3836H | Recombinant Human FAP Protein, GST-tagged | +Inquiry |
|
◆ Lysates
FAP-1881HCL | Recombinant Human FAP cell lysate | +Inquiry |
|
◆ Assay kits
Kit-1729 | FAP Assay Kit | +Inquiry |
|
Easy access to products and services you need from our library via powerful searching tools.
Copyright © 2023 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy