Recombinant Cynomolgus FGL2 protein, His-Avi,Flag-tagged, Biotinylated

• Cat.No.: FGL2-1352C
• Product Overview: Biotinylated Recombinant Cynomolgus FGL2 protein(A0A2K5WID3)(Val205-Pro439), fused with N-terminal His and Avi tag and N-terminal Flag tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&Flag&His
• Protein Length: Val205-Pro439
• Tag: N-His-Avi,N-Flag
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Cynomolgus FGL2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.40 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.