Recombinant Cynomolgus GDF15 protein, hFc-tagged

• Cat.No.: GDF15-6854C
• Product Overview: Recombinant Cynomolgus GDF15 protein(G7PWZ3)(Ala197-Val308), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: Ala197-Val308
• Tag: N-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.