- Cynomolgus monkey(2)
- Rhesus Macaque(1)
Recombinant Cynomolgus GPC3 protein, Fc-tagged
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|Cat.No. :||GPC3-50C Optional Service: Optional requirements on this protein|
|Product Overview :||Recombinant Cynomolgus GPC3 protein (Gln25-His559), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3/GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.|
|Tag :||human IgG1 Fc|
|Form :||Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 87.5 kDa. The protein migrates as 44 kDa, 60 kDa and 100-120 kDa on a SDS-PAGE gel under reducing (R) condition due to naturally cleavage.|
|Protein length :||535|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of GPC3-50C :||
Cynomolgus Glypican 3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of GPC3-50C :||
Immobilized Human FGF basic, Tag Free at 2 μg/mL (100 μL/well) can bind Cynomolgus Glypican 3, Fc Tag with a linear range of 1-31 ng/mL (QC tested).
Immobilized Cynomolgus Glypican 3, Fc Tag at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPC3 Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
|Gene Name :||GPC3|
|Official Symbol :||GPC3|
|Synonyms :||GPC3; glypican 3; glypican-3; OCI-5|
|Gene ID :||102137748|
|mRNA Refseq :||XM_005594608.2|
|Protein Refseq :||XP_005594665.1|
|UniProt ID :||A0A2K5VKD5|
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