| Cat.No. : |
GPC3-50C
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Cynomolgus GPC3 protein (Gln25-His559), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293). |
| Description : |
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3/GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. |
| Source : |
HEK293 |
| Species : |
Cynomolgus |
| Tag : |
human IgG1 Fc |
| Form : |
Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
| Molecular Mass : |
The protein has a calculated MW of 87.5 kDa. The protein migrates as 44 kDa, 60 kDa and 100-120 kDa on a SDS-PAGE gel under reducing (R) condition due to naturally cleavage. |
| Protein length : |
535 |
| Endotoxin : |
Less than 1.0 EU per ug by the LAL method. |
| Purity : |
>90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE of GPC3-50C : |
Cynomolgus Glypican 3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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| Bioactivity-ELISA of GPC3-50C : |
Immobilized Human FGF basic, Tag Free at 2 μg/mL (100 μL/well) can bind Cynomolgus Glypican 3, Fc Tag with a linear range of 1-31 ng/mL (QC tested).
Immobilized Cynomolgus Glypican 3, Fc Tag at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPC3 Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.