Recombinant Cynomolgus IL12A

• Cat.No.: IL12A-190C
• Product Overview: A DNA sequence encoding the cynomolgus IL12A [Identical to the rhesus IL12A (NP_001038199.1)] (Met 1- Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus IL12B [Identical to the

Specification

• Source: Human Cells
• Species: Cynomolgus
• Form: Lyophilized from sterile PBS, pH7.4.
• Molecular Mass: The recombinant cynomolgus IL12A & the cynomolgus IL12B comprises 525(208+317) amino acids and has a calculated molecular mass of 60.2(24+36.2) KDa. The apparent molecular mass of the cynomolgus IL12A & the cynomolgus IL12B heterodimer is approximately 46.1, 42.3 and 36.1 KDa respectively in SDS-PAGE.
• Protein length: Met1-Ser219 & Met1-Ser328
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: (23.1+26.7+49.4) % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.
• Tag: Non

Gene Information

• Gene Name: IL12A?interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [?Macaca mulatta?(Rhesus monkey) ]
• Official Symbol: IL12A
• Synonyms: IL12A; IL-12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; cytotoxic lymphocyte maturation factor 35 kDa subunit
• Gene ID: 703205
• mRNA Refseq: NM_001044734
• Protein Refseq: NP_001038199
• UniProt ID: P48091
• Chromosome Location: 2
• Pathway: African trypanosomiasis; Allograft rejection; Chagas disease (American trypanosomiasis)

Reviews

01/14/2021

Suitable for co-immunoprecipitation.

12/21/2020

Consistent in bioactivity.

11/16/2020

Good for receptor binding studies.

Q&As

How do mass spectrometry-based techniques reveal post-translational modifications on IL12A?

Mass spectrometry identifies IL12A's post-translational modifications and their roles.

What strategies validate IL12A's role in immune response modulation using knockout models?

Knockout models validate IL12A's roles in immune response modulation.

How do protein footprinting assays unveil IL12A's binding interfaces with receptor partners?

Protein footprinting assays reveal IL12A's binding interfaces with receptors.

What experimental methods quantify IL12A's secretion kinetics and stability in cell culture?

ELISA and stability assays quantify IL12A secretion and stability in culture.

Can you discuss the use of SPR in characterizing IL12A's interactions with antibodies and inhibitors?

SPR characterizes IL12A's interactions with antibodies and inhibitors.

What cellular assays assess IL12A's ability to induce cytokine production in immune cells?

Cellular assays gauge IL12A's cytokine induction in immune cells.

How does isothermal titration calorimetry provide insights into IL12A's binding affinity with receptors?

Isothermal titration calorimetry measures IL12A's binding affinity with receptors.

What techniques evaluate the impact of IL12A genetic variants on protein structure and function?

Structural biology techniques analyze IL12A genetic variant impacts.

Could you elaborate on the use of site-directed mutagenesis in probing IL12A's functional domains?

Site-directed mutagenesis probes IL12A's functional domains and interactions.

How does in vivo bioluminescence imaging track IL12A expression dynamics in living organisms?

Bioluminescence imaging tracks IL12A expression dynamics in vivo.