Recombinant Cynomolgus IL1B protein, His-tagged

• Cat.No.: IL1B-1458C
• Product Overview: Recombinant Cynomolgus IL1B protein(A0A2K5VDC7)(Ala117-Ser268), fused with C-terminal His tag, was expressed in E.coli.

Specification

• Species: Cynomolgus
• Source: C-His
• Protein Length: Ala117-Ser268
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS,100mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Human IL-1 RII, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-1 Beta, His Tag with an affinity constant of 1.40 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 18.33 kDa same as Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.